Contact an Account ManagerNucleic acid constituents : 2-dimensional TLC separation of intracellular purine derivatives
Summary
Type: TLC
Application #: 400960
Catalog#: 0422026 - Cellulose 400 TLC Plates, polyester backed, 100um, 20x20cm 25/pk
Detection: autoradiography or UV 254 nm
Conditions: Eluent:
1st dimension: a) methanol (to top of sheet)
b) methanol / n-propanol / 25% aqueous ammonia solution / 1%
aqueous ethylene diamine tetraacetic acid (EDTA) solution
(3:9:2:6, v/v) to middle of sheet, c) as b), but to top of sheet
2nd dimension isobutyric acid / 25% aqueous ammonia solution / 1% aqueous
EDTA solution (200:9:114, v/v) to top of sheet
Authors: Seipel G
Source: J Chromatogr 135 (1977) 485
Details
Legend: O = origin
1. adenine
2. adenosine
3. hypoxanthine
4. inosine
5. guanosine
6. xanthosine
7. xanthine
8. guanine
9. AMP
10. uric acid
11. NAD
12. IMP
13. ADP
14. GMP
15. XMP
16. ATP
17